We are interested in the role of endothelial cells (cells lining the inner surface of blood and lymphatic vessels) in physiological functions and in various diseases, including cancer.
We are studying different growth factors and cytokines on endothelial cell functions, like cell proliferation, migration, tube formation and permeability.
We are investigating endothelial functions on intratumoral angiogenesis and trans-endothelial migration of cancer cells.
We are comparing similar cell functions of the lymphatic endothelial cells, focusing on the differences these cells present from the blood endothelial cells in the in vitro and in vivo context.
We are interested in the role of endothelial cells (cells lining the inner surface of blood and lymphatic vessels) in physiological functions and in various diseases, including cancer. We study the functions of the endothelial cells in various vascular-related diseases and we use this information to provide novel therapeutic approaches. A special focus of the laboratory is to study the role of a protein, the small GTPase RhoA, on endothelial physiology in pathological conditions with a therapeutic perspective.
We have incorporated a repertoire of in vitro and in vivo models to address our questions. Cell migration, tube formation, wound healing, spheroids and live cell imaging are some of the in vitro models used to address the biochemical pathways. In vivo, genetically modified and conditional knockout mice, tumor models, angiogenesis models and live animal imaging through multiphoton microscopy are used for observation of biological processes that cannot be solely addressed at the cellular level.
Constantinos Mikelis
Assistant Professor SOP Biomedical Sciences Amarillo
"The focus of my lab is in the area of vascular biology. A special focus of the lab is to study the role of a protein, the small GTPase RhoA, on endothelial physiology in pathological conditions with a therapeutic perspective."
Ph.D. Principal investigator
Postdoctoral fellow
PharmD Candidate
Alumni
PhD Candidate: Graduated 2020
PhD Candidate: Graduated 2021
PhD Candidate: Graduated 2022
PhD Candidate: Graduated 2022
Marazioti Antonia, PhD
Assistant Professor
Department of Physiotherapy
School of Health Sciences
University of Peloponnese
Greece
1. Nanoparticle Delivery and Tumor Vascular Normalization: The Chicken or The Egg?
Mattheolabakis G, Mikelis CM.
Front Oncol. 2019 Nov 12;9:1227. doi: 10.3389/fonc.2019.01227. eCollection 2019. Review.
Zahra FT, Sajib MS, Ichiyama Y, Akwii RG, Tullar PE, Cobos C, Minchew SA, Doçi CL, Zheng Y, Kubota Y, Gutkind JS, Mikelis CM.
Sci Rep. 2019 Aug 12;9(1):11666
3. In Vitro Wound Healing Assays to Investigate Epidermal Migration.
Main KA, Mikelis CM, Doçi CL.
Methods Mol Biol. 2019 May 24. doi: 10.1007/7651_2019_235. [Epub ahead of print]
4. Role of Angiopoietin-2 in Vascular Physiology and Pathophysiology.
Akwii RG, Sajib MS, Zahra FT, Mikelis CM.
Cells. 2019 May 17;8(5). pii: E471. doi: 10.3390/cells8050471. Review.
5. Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis.
Hossian AKMN, Muthumula CMR, Sajib MS, Tullar PE, Stelly AM, Briski KP, Mikelis CM, Matthaiolampakis G.
J Vis Exp. 2019 Mar 30;(145)
6. Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis.
Kastana P, Zahra FT, Ntenekou D, Katraki-Pavlou S, Beis D, Lionakis MS, Mikelis CM, Papadimitriou E.
Methods Mol Biol. 2019;1952:219-232.
7. In Vitro Spheroid Sprouting Assay of Angiogenesis.
Zahra FT, Choleva E, Sajib MS, Papadimitriou E, Mikelis CM.
Methods Mol Biol. 2019;1952:211-218
8. DNA Repair Response Adaptors: Novel Targets for Vasoproliferative Retinopathy?
Sajib MS, Mikelis CM.
Thromb Haemost. 2019 Mar;119(3):358. doi: 10.1055/s-0038-1677022. Epub 2019 Jan 8.
9. Analogs of penfluridol as chemotherapeutic agents with reduced central nervous system activity.
Ashraf-Uz-Zaman M, Sajib MS, Cucullo L, Mikelis CM, German NA.
Bioorg Med Chem Lett. 2018 Dec 15;28(23-24):3652-3657. doi: 10.1016/j.bmcl.2018.10.036. Epub 2018 Oct 24.
Hossian AKMN, Sajib MS, Tullar PE, Mikelis CM, Mattheolabakis G.
Sci Rep. 2018 Jul 12;8(1):10495.
Punganuru SR, Madala HR, Mikelis CM, Dixit A, Arutla V, Srivenugopal KS.
Oncotarget. 2018 May 25;9(40):26109-26129.
12. GM-CSF Therapy in Human CARD9 Deficiency.
Drummond RA, Zahra FT, Natarajan M, Swamydas M, Hsu AP, Wheat LJ, Gavino C, Vinh DC, Holland SH, Mikelis CM, Lionakis MS. * Co-corresponding author.
J Allergy Clin Immunol. 2018 Jun 8. pii: S0091-6749(18)30846-7.
13. The homozygous CX3CR1-M280 mutation impairs human monocyte survival.
Collar AL, Swamydas M, O’Hayre M, Sajib MS, Hoffman KW, Singh SP, Mourad A, Johnson MD, Ferre EM, Farber JM, Lim JK, Mikelis CM, Gutkind JS, Lionakis MS.
JCI Insight. 2018 Feb 8;3(3). pii: 95417.
14. Mechanisms of angiogenesis in microbe-regulated inflammatory and neoplastic conditions.
Sajib, M.S., Zahra, F.T., Lionakis, M.S., German, N., Mikelis, C.M.
Angiogenesis. 2018 Feb;21(1):1-14. doi: 10.1007/s10456-017-9583-4. Epub 2017 Nov 6
15. Penfluridol induces endoplasmic reticulum stress leading to autophagy in pancreatic cancer.
Ranjan A, German N, Mikelis C, Srivenugopal K, Srivastava SK.
Tumour Biol. 2017 Jun;39(6):1010428317705517.
16. Epidermal loss of Gαq confers a migratory and differentiation defect in keratinocytes.
Doçi CL, Mikelis CM, Callejas-Valera JL, Hansen KK, Molinolo AA, Inoue A, Offermanns S, Gutkind JS. * Co-first author.
PLoS One. 2017 Mar 16;12(3):e0173692. doi: 10.1371/journal.pone.0173692.
17. Angiogenesis in Dental and Oral Conditions: Too important to Ignore.
Sajib, M.S. and Mikelis, C.M. EC Dental Science. 2016 ECP.01: 43-44
18. Extrapulmonary Aspergillus
Rieber N, Gazendam RP, Freeman AF, Hsu AP, Collar AL, Sugui JA, Drummond RA, Rongkavilit C, Hoffman K, Henderson C, Clark L, Mezger M, Swamydas M, Engeholm M, Schüle R, Neumayer B, Ebel F, Mikelis CM, Pittaluga S, Prasad VK, Singh A, Milner JD, Williams KW, Lim JK, Kwon-Chung KJ, Holland SM, Hartl D, Kuijpers TW, Lionakis MS.
JCI Insight. 2016 Oct 20;1(17):e89890.
Drummond RA, Collar AL, Swamydas M, Rodriguez CA, Lim JK, Mendez LM, Fink DL, Hsu AP, Zhai B, Karauzum H, Mikelis CM, Rose SR, Ferre EM, Yockey L, Lemberg K, Kuehn HS, Rosenzweig SD, Lin X, Chittiboina P, Datta SK, Belhorn TH, Weimer ET, Hernandez ML, Hohl TM, Kuhns DB, Lionakis MS.
PLoS Pathog. 2015 Dec 17;11(12):e1005293. doi: 10.1371/journal.ppat.1005293. eCollection 2015 Dec.
O’Hayre M, Inoue A, Kufareva I, Wang Z, Mikelis CM, Drummond RA, Avino S, Finkel K, Kalim KW, DiPasquale G, Guo F, Aoki J, Zheng Y, Lionakis MS, Molinolo AA, Gutkind JS.
Oncogene. 2015 Nov 30. doi: 10.1038/onc.2015.442.
Punganuru SR, Madala HR, Venugopal SN, Samala R, Mikelis C, Srivenugopal KS.
Eur J Med Chem. 2016 Jan 1;107:233-44. doi: 10.1016/j.ejmech.2015.10.052. Epub 2015 Nov 2.
Doçi CL, Mikelis CM, Lionakis MS, Molinolo AA, Gutkind JS.
Cancer Res. 2015 Jul 15;75(14):2937-48. doi: 10.1158/0008-5472.CAN-14-3121. Epub 2015 May 7.
23. RhoA and ROCK mediate histamine-induced vascular leakage and anaphylactic shock.
Mikelis CM, Simaan M, Ando K, Fukuhara S, Sakurai A, Amornphimoltham P, Masedunskas A, Weigert R, Chavakis T, Adams RH, Offermanns S, Mochizuki N, Zheng Y, Gutkind JS.
Nat Commun. 2015 Apr 10;6:6725. doi: 10.1038/ncomms7725.
Dillenburg-Pilla P, Patel V, Mikelis CM, Zárate-Bladés CR, Doçi CL, Amornphimoltham P, Wang Z, Martin D, Leelahavanichkul K, Dorsam RT, Masedunskas A, Weigert R, Molinolo AA, Gutkind JS.
FASEB J. 2015 Mar;29(3):1056-68. doi: 10.1096/fj.14-260083. Epub 2014 Dec 2.
25. Heterotrimeric G-protein alpha-12 (Gα12) subunit promotes oral cancer metastasis.
Gan CP, Patel V, Mikelis CM, Zain RB, Molinolo AA, Abraham MT, Teo SH, Abdul Rahman ZA, Gutkind JS, Cheong SC.
Oncotarget. 2014 Oct 30;5(20):9626-40.
26. CX3CR1-dependent renal macrophage survival promotes Candida control and host survival.
Lionakis MS, Swamydas M, Fischer BG, Plantinga TS, Johnson MD, Jaeger M, Green NM, Masedunskas A, Weigert R, Mikelis C, Wan W, Lee CC, Lim JK, Rivollier A, Yang JC, Laird GM, Wheeler RT, Alexander BD, Perfect JR, Gao JL, Kullberg BJ, Netea MG, Murphy PM.
J Clin Invest. 2013 Dec;123(12):5035-51. doi: 10.1172/JCI71307. Epub 2013 Nov 1.
Chen JJ, Mikelis CM, Zhang Y, Gutkind JS, Zhang B.
Oncotarget. 2013 Feb;4(2):206-17.
Mikelis CM, Palmby TR, Simaan M, Li W, Szabo R, Lyons R, Martin D, Yagi H, Fukuhara S, Chikumi H, Galisteo R, Mukouyama YS, Bugge TH, Gutkind JS.
J Biol Chem. 2013 Apr 26;288(17):12232-43. doi: 10.1074/jbc.M112.428599. Epub 2013 Mar 6.
Kaspiris A, Mikelis C, Heroult M, Khaldi L, Grivas TB, Kouvaras I, Dangas S, Vasiliadis E, Lioté F, Courty J, Papadimitriou E.
Joint Bone Spine. 2013 Jul;80(4):407-13. doi: 10.1016/j.jbspin.2012.10.024. Epub 2013 Jan 18.
30. Interplay between αvβ3 integrin and nucleolin regulates human endothelial and glioma cell migration.
Koutsioumpa M, Polytarchou C, Courty J, Zhang Y, Kieffer N, Mikelis C, Skandalis SS, Hellman U, Iliopoulos D, Papadimitriou E.
J Biol Chem. 2013 Jan 4;288(1):343-54. doi: 10.1074/jbc.M112.387076. Epub 2012 Nov 16.
Pan W, Pham VN, Stratman AN, Castranova D, Kamei M, Kidd KR, Lo BD, Shaw KM, Torres-Vazquez J, Mikelis CM, Gutkind JS, Davis GE, Weinstein BM.
Blood. 2012 Jul 12;120(2):489-98. doi: 10.1182/blood-2012-02-408328. Epub 2012 May 30.
Koutsioumpa M, Drosou G, Mikelis C, Theochari K, Vourtsis D, Katsoris P, Giannopoulou E, Courty J, Petrou C, Magafa V, Cordopatis P, Papadimitriou E.
Vasc Cell. 2012 Mar 16;4:4. doi: 10.1186/2045-824X-4-4.
33. Decreased lymphangiogenesis and lymph node metastasis by mTOR inhibition in head and neck cancer.
Patel V, Marsh CA, Dorsam RT, Mikelis CM, Masedunskas A, Amornphimoltham P, Nathan CA, Singh B, Weigert R, Molinolo AA, Gutkind JS.
Cancer Res. 2011 Nov 15;71(22):7103-12. doi: 10.1158/0008-5472.CAN-10-3192. Epub 2011 Oct 5. Erratum in: Cancer Res. 2012 Feb 1;72(3):826. Nathan, Cherie Ann [corrected to Nathan, Cherie-Ann O].
Mikelis C, Lamprou M, Koutsioumpa M, Koutsioubas AG, Spyranti Z, Zompra AA, Spiliopoulos N, Vradis AA, Katsoris P, Spyroulias GA, Cordopatis P, Courty J, Papadimitriou E.
J Cell Biochem. 2011 Jun;112(6):1532-43. doi: 10.1002/jcb.23066.
35. Differential endothelial transcriptomics identifies semaphorin 3G as a vascular class 3 semaphorin.
Kutschera S, Weber H, Weick A, De Smet F, Genove G, Takemoto M, Prahst C, Riedel M, Mikelis C, Baulande S, Champseix C, Kummerer P, Conseiller E, Multon MC, Heroult M, Bicknell R, Carmeliet P, Betsholtz C, Augustin HG.
Arterioscler Thromb Vasc Biol. 2011 Jan;31(1):151-9. doi: 10.1161/ATVBAHA.110.215871. Epub 2010 Oct 14.
36. Roles of pleiotrophin in tumor growth and angiogenesis.
Papadimitriou E, Mikelis C, Lampropoulou E, Koutsioumpa M, Theochari K, Tsirmoula S, Theodoropoulou C, Lamprou M, Sfaelou E, Vourtsis D, Boudouris P.
Eur Cytokine Netw. 2009 Dec;20(4):180-90. doi: 10.1684/ecn.2009.0172. Review.
Mikelis C, Sfaelou E, Koutsioumpa M, Kieffer N, Papadimitriou E.
FASEB J. 2009 May;23(5):1459-69. doi: 10.1096/fj.08-117564. Epub 2009 Jan 13.
Koutsioumpa M, Hatziapostolou M, Mikelis C, Koolwijk P, Papadimitriou E.
Eur J Pharmacol. 2009 Jan 14;602(2-3):245-9. doi: 10.1016/j.ejphar.2008.11.046. Epub 2008 Dec 3.
Polytarchou C, Hatziapostolou M, Poimenidi E, Mikelis C, Papadopoulou A, Parthymou A, Papadimitriou E.
Int J Cancer. 2009 Apr 15;124(8):1785-93. doi: 10.1002/ijc.24084.
40. Heparin-binding protein pleiotrophin: an important player in the angiogenic process.
Mikelis C, Papadimitriou E.
Connect Tissue Res. 2008;49(3):149-52. doi: 10.1080/03008200802148652. Review.
41. Pleiotrophin as a possible new target for angiogenesis-related diseases and cancer.
Mikelis C, Koutsioumpa M, Papadimitriou E.
Recent Pat Anticancer Drug Discov. 2007 Jun;2(2):175-86. Review.
Parthymou A, Lampropoulou E, Mikelis C, Drosou G, Papadimitriou E.
Eur J Cell Biol. 2008 Jan;87(1):17-29. Epub 2007 Sep 18.
Kokolakis G, Mikelis C, Papadimitriou E, Courty J, Karetsou E, Katsoris P.
In Vivo. 2006 Sep-Oct;20(5):629-35.
44. Heparin affin regulatory peptide: a new target for tumour therapy?
Papadimitriou E, Polykratis A, Hatziapostolou M, Parthymou A, Polytarchou C, Mikelis C.
Curr Cancer Drug Targets. 2004 Sep;4(6):471-82. Review.
See updated publication list on PubMed: Click here
Honors and Awards of the members of the Mikelis lab
– K Wyatt McMahon Outstanding Graduate Student Award, GSBS Graduation Ceremony, May 2022: Fatema Zahra
– 2022 Dean’s Scholar Award: Racheal Akwii
– 2022 Travel Award for the ASPET Annual Meeting at Experimental Biology (EB): Behnaz Lahooti
– 2022 TTUHSC School of Pharmacy Graduate Mentor of the Year Award: Constantinos Mikelis
– K Wyatt McMahon Outstanding Graduate Student Award, GSBS Graduation Ceremony, May 7 2021: Sanaullah Sajib
– 2021 Phonathon Scholarship: Fatema Zahra
– Selection for the NCI Graduate Student Recruiting Program, May 4-5 2021, Bethesda, MD: Fatema Zahra
– ASIP 2021 Experimental Pathologist-in-Graduate-Training (EPIGT) Award: Fatema Zahra
– 2021 Dean’s Scholarship Award, and for the duration of her studies at TTUHSC: Racheal Akwii
– Best Oral Presentation in 2021 Graduate Student Seminars, Graduate Program in Pharmaceutical Sciences, December 2021: Racheal Akwii
– 2021 Mai Anh Do & David Nguyen Foundation Scholarship: Behnaz Lahooti
– 2021 Mary Lou Clements-Mann Scholarship: Behnaz Lahooti
– 2021 Scholarship of American Association of Iranian Pharmaceutical Scientists: Behnaz Lahooti
– 2021 TTUHSC School of Pharmacy Graduate Teacher of the Year Award: Constantinos Mikelis
– 2021 Faculty Appreciation Award for the TTUHSC Graduate School of Biomedical Sciences, sponsored by the Student Senate: Constantinos Mikelis
– 2021 TTUHSC Graduate School of Biomedical Sciences Outstanding Teacher of the Year Award, sponsored by the Student Senate: Constantinos Mikelis
– Graduate Student Travel Support Award for the Society of Toxicology 59th Annual Meeting in Anaheim, California, March 15-19, 2020: Fatema Zahra
– Selection by the American Society for Investigative Pathology (ASIP) for Oral Presentation at the Experimental Biology meeting in San Diego, California, April 4-7, 2020: Fatema Zahra
– Selection for Podium Presentation in 32nd Annual TTUHSC Student Research week, March 10-13 2020, Lubbock, TX: Fatema Zahra
– 2020 Dean’s Recognition Award: Racheal Akwii
– 2020 Dean’s Scholarship Award, and for the duration of her studies at TTUHSC: Fatema Zahra
– 2020 AAPS Best Abstract Award, American Association of Pharmaceutical Scientists, AAPS PharmSci 360, October 26-November 5, New Orleans, LA: Fatema Zahra
– PISA 2020 GALL Trainee Scholar Award for Excellence in Cardiovascular Research, PISA 2020 Meeting, November 9-13 2020 Virtual Conference: Fatema Zahra
– 2020 Dean’s Recognition Award: Racheal Akwii
– 2020 TTUHSC Graduate School of Biomedical Sciences Outstanding Faculty Award, sponsored by the Student Senate: Constantinos Mikelis
– 2020 TTUHSC Graduate School of Biomedical Sciences Outstanding Teacher of the Year Award, sponsored by the Student Senate: Constantinos Mikelis
– 2020 TTUHSC Graduate School of Biomedical Sciences Outstanding Teacher of the Year Award, sponsored by the Student Senate: Constantinos Mikelis
– 2020 TTUHSC School of Pharmacy Graduate Mentor of the Year Award: Constantinos Mikelis
– 2020 TTUHSC School of Pharmacy Graduate Mentor of the Year Award: Constantinos Mikelis
– 2020 TTUHSC School of Pharmacy Graduate Teacher of the Year Award: Constantinos Mikelis
– TTUHSC School of Pharmacy Graduate Teacher of the Year Award: Constantinos Mikelis
– TTUHSC School of Pharmacy Graduate Mentor of the Year Award: Constantinos Mikelis
– TTUHSC Graduate School of Biomedical Sciences Outstanding Teacher of the Year Award, sponsored by the Student Senate: Constantinos Mikelis
– 2018-2019 American Association of University Women (AAUW) International Fellowship: Racheal Akwii
– American Society for Biochemistry and Molecular Biology (ASBMB) Graduate Student Travel Award, 2019 Experimental Biology Meeting, April 6-9, Orlando, Florida: Md Sanaullah Sajib
– Selection for the NCI Graduate Student Recruiting Program, May 7-8 2019, Bethesda, MD: Md Sanaullah Sajib
– Third poster award in 31st Annual TTUHSC Student Research week, March 20-22 2019, Lubbock, TX: Md Sanaullah Sajib
– American Society for Biochemistry and Molecular Biology (ASBMB) Graduate Student Travel Award, 2019 Experimental Biology Meeting, April 6-9, Orlando, FL: Fatema Tuz Zahra
– Cayman Chemical Travel Award for attendance of the 2019 Experimental Biology Meeting, April 6-9, Orlando, FL: Fatema Tuz Zahra
– Vasculata Scholarship, for attendance of 2019 Vasculata, July 15-18, Medical College of Wisconsin, Milwaukee, WI: Fatema Tuz Zahra
– 2019 Mary Lou Clements-Mann Scholarship: Fatema Tuz Zahra
– Poster Award in 2019 Gordon Research Seminar on Angiogenesis, August 3-4, Newport, Rhode Island, CT: Fatema Tuz Zahra
– Graduate Student Travel Support Award for the Society of Toxicology 59th Annual Meeting in Anaheim, California, March 15-19, 2020: Fatema Tuz Zahra
– Best Oral Presentation in 2019 Graduate Student Seminars, Graduate Program in Pharmaceutical Sciences, September 4th 2019: Racheal Akwii
– Graduate Student Travel Support Award, 58th Annual Meeting of the Society of Toxicology, March 10-14 2019, Baltimore, MD: Md Sanaullah Sajib
– Young Investigator Fellowship Award, Symposia on Cancer Research 2018, October 4-5, MD Anderson Cancer Center, Houston, TX: Md Sanaullah Sajib
– 2018 Phonathon Scholarship: Md Sanaullah Sajib
– Selection for Podium Presentation by TTUHSC Graduate School of Biomedical Sciences at 2018 Student Research Week (SRW), March 20-25, Lubbock, Texas: Md Sanaullah Sajib
– 2018 Vasculata Scholarship Award for the 2018 Vasculata, July 23-26, Washington University, St. Louis, MO: Fatema Tuz Zahra
– 2018 Vasculata Scholarship Award for the 2018 Vasculata, July 23-26, Washington University, St. Louis, MO: Sanaullah Sajib
– Selection for Podium Presentation at the 17th Amarillo Research Days, Amarillo, TX, June 13-14, 2018: Fatema Tuz Zahra
– 2018 Mary Lou Clements-Mann Scholarship: Fatema Tuz Zahra
– Best Oral Presentation in 2018 Graduate Student Seminars, Graduate Program in Pharmaceutical Sciences, September 4th 2018: Fatema Tuz Zahra
– “Graduate Mentor of the Year” award for the School of Pharmacy, TTUHSC (2017): Constantinos Mikelis
– “Outstanding Teacher of the Year” award for the Graduate School of Biomedical Sciences, sponsored by the Student Senate (2017): Constantinos Mikelis
– 2017 AAPS BIOTEC Section Travel Award for the AAPS National Biotechnology Conference, May 1-3 2017, San Diego, California: Md Sanaullah Sajib
– Best Podium Presentation Award during the 16 th Annual Amarillo Research Days, June 15-16 2017, Amarillo, Texas: Md Sanaullah Sajib
– 2017 Vasculata Scholarship Award for the 2017 Vasculata, July 24-27, Chicago, Illinois: Md Sanaullah Sajib
– 2017 Vasculata Scholarship Award for the 2017 Vasculata, July 24-27, Chicago, Illinois: Fatema Tuz Zahra
– 2017 Mary Lou Clements-Mann Scholarship: Fatema Tuz Zahra
– 2017 Mary Lou Clements-Mann Scholarship: Md Sanaullah Sajib
– Best Oral Presentation in 2017 Graduate Student Seminars, Graduate Program in Pharmaceutical Sciences, September 1, 2017: Md Sanaullah Sajib
" in action "
Contact information
Contact
Constantinos Mikelis, Ph.D.
Associate Professor
Department of Pharmacy
University of Patras
Tel: +30 2610 962362
Office - Lab
Laboratory of Molecular Pharmacology
Department of Pharmacy
New Pharmacy Building
University of Patras
Patras, Greece
26504